IMI2 Programme
He holds degrees in Chemistry (Univ. Ramon Llull,1994) and Chemical Engineering (IQS, 1995). After obtaining a Ph D in Organic Chemistry (University of Barcelona, 1999) he joined the group of Lewis Kay in Toronto for a post-doctoral stay (University of Toronto, 2000-2004). He was then recipient of a Ramon y Cajal reincorporation contract at the Parc Cientific de Barcelona (2004-2006) and he currently is group leader at the CIC bioGUNE. His research line focuses of the use of nuclear magnetic resonance (NMR) to the study of biologically relevant proteins and enzymes, paying special attention to the delicate balance existing between protein stability and dynamics. Such knowledge is applied for the development of new compounds with therapeutic activity, specifically in the field of rare diseases. He have published more than 55 papers with a total number of citations (1998-2015) of 2050 and an h-index of 17. He was the Spanish delegate for the trans domain of the COST program (2009-2014). He was awarded the prize of the Real Sociedad Española de Química (2004) and the Spanish NMR group prize (2005). He currently is the president of the Spanish NMR group.
Latest Publications
2023
2022
Protein stability (thermodynamic and kinetic) drives the biophysical properties of the polypeptide chain (protein folding) and the protein's concentration in the cellular environment (protein homeostasis). It is the result of a delicate balance between inter- and intramolecular interactions, which can be easily altered by mutations and/or upon changes in the composition of the surrounding media. In this context, NMR spectroscopy offers a plethora of suitable experiments to investigate protein stability. In our laboratory we are currently interested in the following topics:
-
Pharmacological chaperones. Rare diseases (~7000 identified to date) are an area of significant medical need affecting an estimated 350 million people worldwide, with ~95% having no currently approved drug treatment. They are often produced by inherited mutations affecting the activity of a protein and It is becoming increasily clear that, most frequently, a mutation destabilizes the protein/enzyme, ultimately affecting its intracellular homeostasis. In this context, pharmacological chaperones (small molecules which bind to the protein, restoring stability and activity without affecting its function) can be applied to many diseases. In our laboratory we are investigating new methods (NMR, biophysical and biochemical) for the discovery and characterization of pharmacological chaperones against a set of diseases: congenital eryhtropoietic porphyria, tyrosianemia.
- Environmental modulation of enzyme stability. The high catalytic efficiency and the exquisite enantioselectivity of an enzyme has been employed in some industrial processes to upgrade their properties in order to make them more environmentally-friendly. However, large scale industrial implementation of biotechnological reactions is often limited by the marginal stability of the enzyme in the reactor conditions. In our laboratory we employ NMR and circular dichroism to investigate the effect of external crowding agents to improve the activity and stability of several enzymes. Specifically, we are investigating the mechanism for protein haloadaptation by a combined use of site directed mutagenesis and high-resolution NMR spectroscopy.
-
General Director
José M Mato
IMI2 Programme Emerging Scientist -
Gabriel Ortega Quintanilla
Ikerbasque Research Fellow - Ramón y Cajal (RyC) Programme RESEARCH ASSISTANT -
Nieves Embade
-
Fernando Lopitz Otsoa
SPECIALIST -
Beatriz González Valle
-
Rubén Gil Redondo
POSTDOCTORAL RESEARCHER -
Alain Ibañez de Opakua Lopez de Abetxuko
-
David Fernández Ramos
-
Andreas Schedlbauer
-
Ganeko Bernardo
TECHNICIANS / DOCTORAL CANDIDATES -
Alba Pejenaute Pejenaute
-
Riccardo Scarin
-
Uxue Hermoso Martínez
-
Laura Gálvez Larrosa
-
Maider Bizkarguenaga Uribiarte
-
Maria de los Reyes Luque Urbano
-
Tania Pereira Ortuzar
-
Sara Martín Ramos
-
Pablo Herrero Alfonso
-
Ángela de Diego Rodríguez
-
Margarita Gómez Galán
-
Ricardo Diogo Alves Conde
-
Ana Laín
-
Karen Salua Alarcón Morales
-
Jon Gil Martinez
-
Cristina Garrido
-
Virginia Gutiérrez de Juan
Members
Latest Publications
Stratification of Sepsis Patients on Admission into the Intensive Care Unit According to Differential Plasma Metabolic Phenotypes
Lodge, S; Litton, E; Gray, N; Ryan, M; Millet, O; Fear, M; Raby, E; Currie, A; Wood, F; Holmes, E; Wist, J; Nicholson, JK;
JOURNAL OF PROTEOME RESEARCH
2024-03-21
Cross-Validation of Metabolic Phenotypes in SARS-CoV-2 Infected Subpopulations Using Targeted Liquid Chromatography-Mass Spectrometry (LC-MS)
Whiley, L; Lawler, NG; Zeng, AX; Lee, A; Chin, ST; Bizkarguenaga, M; Bruzzone, C; Embade, N; Wist, J; Holmes, E; Millet, O; Nicholson, JK; Gray, N;
JOURNAL OF PROTEOME RESEARCH
2024-03-14
Characterization of preovulatory follicular fluid secretome and its effects on equine oocytes during in vitro maturation.
Luis-Calero, Marcos; Marinaro, Federica; Fernandez-Hernandez, Pablo; Ortiz-Rodriguez, Jose M; G Casado, Javier; Pericuesta, Eva; Gutierrez-Adan, Alfonso; Gonzalez, Esperanza; Azkargorta, Mikel; ...
Research in veterinary science
2024-03-11
Impaired Hepatic Very Low-Density Lipoprotein Secretion Promotes Tumorigenesis and Is Accelerated with Fabp1 Deletion.
Newberry, Elizabeth P; Molitor, Elizabeth A; Liu, Allen; Chong, Kamyar; Liu, Xiuli; Alonso, Cristina; Mato, Jose M; Davidson, Nicholas O;
The American journal of pathology
2024-02-28
Serum and Urine Metabolomic Profiling of Newly Diagnosed Treatment-Naive Inflammatory Bowel Disease Patients
Aldars-García, L; Gil-Redondo, R; Embade, N; Riestra, S; Rivero, M; Gutiérrez, A; Rodríguez-Lago, I; Fernández-Salazar, L; Ceballos, D; Benitez, JM; Aguas, M; Baston-Rey, I; Bermejo, F; Casanova, ...
INFLAMMATORY BOWEL DISEASES
2024-02-01
Quantitative Analysis of the Human Semen Phosphorometabolome by 31P-NMR
Serrano, R; Martin-Hidalgo, D; Bilbao, J; Bernardo-Seisdedos, G; Millet, O; Garcia-Marin, LJ; Bragado, MJ;
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2024-02-01
Effect of age and dietary habits on Red Blood Cell membrane fatty acids in a Southern Europe population (Basque Country)
Marrugat, G; Cano, A; Amézaga, J; Arranz, S; Embade, N; Millet, O; Ferreri, C; Tueros, I;
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS
2024-01-01
Serum identification of at-risk MASH: The metabolomics-advanced steatohepatitis fibrosis score (MASEF)
Noureddin, M; Truong, E; Mayo, R; Martínez-Arranz, I; Banales, JM; Mincholé, I; Arrese, M; Cusi, K; Arias-Loste, MT; Bruha, R; Romero-Gómez, M; Iruzubieta, P; Aller, R; Ampuero, J; Calleja, ...
HEPATOLOGY
2024-01-01
S-Adenosylmethionine Negatively Regulates the Mitochondrial Respiratory Chain Repressor MCJ in the Liver
Barbier-Torres, L; Chhimwal, J; Kim, SY; Ramani, K; Robinson, A; Yang, HP; Van Eyk, J; Liangpunsakul, S; Seki, E; Mato, JM; Lu, SC;
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
2024-01-01
Hepatic prohibitin 1 and methionine adenosyltransferase a1 defend against primary and secondary liver cancer metastasis
Fan, W; Cao, DY; Yang, B; Wang, JH; Li, XM; Kitka, D; Li, TWH; You, SY; Shiao, S; Gangi, A; Posadas, E; Di Vizio, D; Tomasi, ML; Seki, E; Mato, JM; Yang, HP; Lu, SC;
JOURNAL OF HEPATOLOGY
2023-12-10
Hepatocyte-specific O-GlcNAc transferase downregulation ameliorates nonalcoholic steatohepatitis by improving mitochondrial function
Gonzalez-Rellan, MJ; Parracho, T; Heras, V; Rodriguez, A; Fondevila, MF; Novoa, E; Lima, N; Varela-Rey, M; Senra, A; Chantada-Vazquez, MDP; Ameneiro, C; Bernardo, G; Fernandez-Ramos, D; Lopitz-Otsoa, ...
MOLECULAR METABOLISM
2023-09-01
Hepatic levels of S-adenosylmethionine regulate the adaptive response to fasting
Capelo-Diz, A; Lachiondo-Ortega, S; Fernández-Ramos, D; Cañas-Martín, J; Goikoetxea-Usandizaga, N; Serrano-Maciá, M; González-Rellan, MJ; Mosca, L; Blazquez-Vicens, J; Tinahones-Ruano, A; Fondevila, ...
CELL METABOLISM
2023-08-08
Performance of non-invasive tests and histology for the prediction of clinical outcomes in patients with non-alcoholic fatty liver disease: an individual participant data meta-analysis
Mózes, FE; Lee, JA; Vali, Y; Alzoubi, O; Staufer, K; Trauner, M; Paternostro, R; Stauber, RE; Holleboom, AG; van Dijk, AM; Mak, AL; Boursier, J; Loup, MD; Shima, T; Bugianesi, E; Gaia, S; Armandi, ...
Lancet Gastroenterology & Hepatology
2023-08-01
Integrative Plasma Metabolic and Lipidomic Modelling of SARS-CoV-2 Infection in Relation to Clinical Severity and Early Mortality Prediction
Lodge, S; Lawler, NG; Gray, N; Masuda, R; Nitschke, P; Whiley, L; Bong, SH; Yeap, BB; Dwivedi, G; Spraul, M; Schaefer, H; Gil-Redondo, R; Embade, N; Millet, O; Holmes, E; Wist, J; Nicholson, ...
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2023-07-01
Urinary Metabolomic Profile of Youth at Risk of Chronic Kidney Disease in Nicaragua
Hall, SM; Raines, NH; Ramirez-Rubio, O; Amador, JJ; López-Pilarte, D; OCallaghan-Gordo, C; Gil-Redondo, R; Embade, N; Millet, O; Peng, XJ; Vences, S; Keogh, SA; Delgado, IS; Friedman, DJ; Brooks, ...
KIDNEY360
2023-07-01
Metabolic Features of Increased Gut Permeability, Inflammation, and Altered Energy Metabolism Distinguish Agricultural Workers at Risk for Mesoamerican Nephropathy
Raines, NH; Leone, DA; OCallaghan-Gordo, C; Ramirez-Rubio, O; Amador, JJ; Pilarte, DL; Delgado, IS; Leibler, JH; Embade, N; Gil-Redondo, R; Bruzzone, C; Bizkarguenaga, M; Scammell, MK; Parikh, ...
METABOLITES
2023-03-01
Redox regulation of KV7 channels through EF3 hand of calmodulin
Nunez, E; Jones, F; Muguruza-Montero, A; Urrutia, J; Aguado, A; Malo, C; Bernardo-Seisdedos, G; Domene, C; Millet, O; Gamper, N; Villarroel, A; Colecraft, HM;
ELIFE
2023-02-20
Hyperphosphorylation of hepatic proteome characterizes nonalcoholic fatty liver disease in S-adenosylmethionine deficiency
Robinson, AE; Binek, A; Ramani, K; Sundararaman, N; Barbier-Torres, L; Murray, B; Venkatraman, V; Kreimer, S; Mc Ardle, A; Noureddin, M; Fernández-Ramos, D; Lopitz-Otsoa, F; de Juan, VG; Millet, ...
ISCIENCE
2023-02-17
Metabolomics as a powerful tool for diagnostic, pronostic and drug intervention analysis in COVID-19
Bruzzone, C; Conde, R; Embade, N; Mato, JM; Millet, O;
FRONTIERS IN MOLECULAR BIOSCIENCES
2023-02-15
Impaired Function of Solute Carrier Family 19 Leads to Low Folate Levels and Lipid Droplet Accumulation in Hepatocytes
Cano, A; Vazquez-Chantada, M; Conde-Vancells, J; Gonzalez-Lahera, A; Mosen-Ansorena, D; Blanco, FJ; Clément, K; Aron-Wisnewsky, J; Tran, A; Gual, P; García-Monzón, C; Caballería, J; Castro, ...
BIOMEDICINES
2023-02-01